Dynamic changes in zeatin nucleoside (ZR) content of mango cutback fruit branch.
Peng Lei, Zhou Ling, Wang Ying, Gao XiaoJun, Long WenHong, Wu XingEn, Li TingTing, Liu Juan
Author Affiliation: College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China.
Journal of Southern Agriculture 44 : 39-42
Abstract : Objective: The zeatin nucleoside (ZR) content in various organs of mango fruiting branches during the flower bud differentiation period was determined in order to explore the role of ZR in mango flower bud differentiation. Method: Utilizing the phenomenon of re-blossoming and re-fruiting in mango pruning buds after cutting back the fruit branches, the enzyme-linked immunosorbent assay method (ELISA) was applied to determine the ZR contents in pruning leaf, phloem, and buds. Result: After pruning the fruiting branches, the ZR contents of pruning leaf, phloem, and pruning buds reached minimum at 1.73, 1.52, and 2.0 µg.g-1 FW on February 25, February 20, and March 2, respectively. The ZR contents of pruning leaf, phloem, and buds reached maximum at 6.39, 8.47, and 10.32 µg.g-1 FW respectively on March 12. On February 25, the color of the central heart area in pruning buds gradually turned sallow and proliferated around the shoot area. From March 2 to March 7, the buds gradually dilated, sallow color deepened, and the central heart area appeared translucent. After March 7, green color expanded and the translucent appearance disappeared. Conclusion: The ZR exhibited different roles at different stages of mango fruit flower bud differentiation. During the critical period of flower bud differentiation, the ZR content was low and the cell activity growth decreased, which were beneficial qualities for mango plants to transform from the bud physiological state into the flower bud physiological state. After the critical period of flower bud differentiation, relatively high ZR content was advantageous for the flower bud differentiation process in mango plant growth.