Isolation, identification and diversity analysis of endophytic fungi in Mangifera indica.
Wu JingBo, Zhan RuLin, Liu Feng, Li GuoPing, Zhao YanLong, Chang JinMei
Author Affiliation: College of Environment and Plant Protection, Hainan University, Haikou, Hainan 571737, China.
Journal of Fruit Science 30 : 1030-1039
Abstract : Objective: The objective of the study were to analyze the composition, diversity and distribution of endophytic fungal community of Mangifera indica Linn (Mango). Method: The endophytic fungi isolated from the four kinds of mango were identified and classified by the morphological observation and ITS sequence analysis. The result were analyzed by the indexes such as isolation rate (IR), colonization rate (CR), relative frequency (RF), Shannon-Wiener biodiversity index (H?) and similarity coefficient (Cs). Result: Fifty three strains of endophytic fungi were isolated from the 480 pieces of mango organizations. They were classified and identified to 16 taxa, 14 species of Ascomycota and 2 species of Basidiomycota were obtained. The dominant groups of those endophytic strains were Colletotrichum gloeosporioides, Guignardia mangiferae and Botryosphaeria dothidea, accounted for 20.75%, 18.87% and 18.87%, respectively. Conclusion: The composition of endophytic fungal community in mango has a certain diversity, and the distribution of endophytic fungi has the species and tissue specificity.