Analysis by RP-HPLC of mangiferin component correlation between medicinal loranthus and their mango host trees.
Zhang XieJun, Su BenWei, Li Jing, Li YongHua, Lu Dong, Zhu KaiXin, Pei HeHuan, Zhao MingHui
Author Affiliation: Guangxi Traditional Chinese Medicine University, Nanning, 530001, China.
Journal of Chromatographic Science 52 : 1-4
Abstract : This study proposes a reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of mangiferin in medicinal loranthus shrubs and their mango or non-mango host trees. Mangiferin in samples was extracted with a solution of 40% methanol. Analytical determination was conducted by RP-HPLC with ultraviolet detection at 258 nm. Chromatographic separation was achieved on an Inertsil ODS-SP column (250×4.6 mm, 5 µm) by isocratic elution with methanol-0.1% aqueous phosphoric acid (31:69, v/v). Mangiferin contents were 5.04 to 18.95 mg/g in mango trees and 0.44 to 3.72 mg/g in medicinal loranthus parasitized on mango host trees. Mangiferin could not be detected in non-mango trees and their loranthus shrubs. This study indicated that host trees could affect the quality of medicinal loranthus by transporting host-inherent components into loranthus.