Ultrasound-assisted extraction of Mangiferin from mango (Mangifera indica L.) leaves using response surface methodology.
Zou TangBin, Xia EnQin, He TaiPing, Huang MingYuan, Jia Qing, Li HuaWen
Author Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Guangdong Medical College, Dongguan 523808, China.
Molecules 19 : 1411-1421
Abstract : Mangiferin is a xanthone widely distributed in higher plants showing antioxidative, antiviral, anticancer, antidiabetic, immunomodulatory, hepatoprotective and analgesic effects. In the present study, an ultrasonic-assisted extraction method was developed for the effective extraction of mangiferin from mango leaves. Some parameters such as ethanol concentration, liquid-to-solid ratio, extraction temperature, and extraction time were optimized by single-factor experiment and response surface methodology. The optimal extraction conditions were 44% ethanol, the liquid-to-solid ratio was 38:1, and extraction for 19.2 min at 60°C under ultrasound irradiation of 200 W. Under optimal conditions, the yield of mangiferin was 58.46±1.27 mg/g. The results obtained are helpful for the full utilization of mango leaves, and also indicated that ultrasonic-assisted extraction is a very useful method for the extraction of mangiferin from plant materials.