Molecular characterization of Fusarium moniliforme var. subglutinans isolates.
Pradeep Kumar, Gupta V. K., Misra A. K., Modi D. R.
Author Affiliation: The Department of Biotechnology Engineering, Ben Gurion University of The Negev, Be'er Sheva-84105, Israel.
Journal of Environmental Biology 35 : 211-216
Abstract : Molecular characterization of 42 isolates of mango (Mangifera indica L.) malformation pathogen Fusarium moniliforme var. subglutinans collected from different agro-climatic zones of India were performed using RAPD markers. Twenty RAPD primers of OPA series were selected for screening of which; six primers OPA-1, 3, 7, 8, 9 and 18 were found to be clearly discriminates and differentiated the F. moniliforme var. subglutinans isolates and showed reproducible banding patterns. A sum total of 598 bands were generated from these random primers having range from 4.0% to 90.9% of similarity among the 42 isolates of F. moniliforme var. subglutinans. The data were used to construct a dendrogram with unweighted pair group method of arithmetical averages (UPGMA) algorithm, using the NTSYS-software. Dendogram revealed more than 90% similarity among all the 42 isolates which shows that all isolates approximately have same genetic identity. Hence, in the current research findings RAPD proved a reproducible and tractable means of differentiating F. moniliforme var. subglutinans isolates.