Evaluation of antibacterial and phytochemical analysis of Mangifera indica bark extracts.
Ashok V. G., Priya S. B., Pranita A. G.
Author Affiliation: Department of Microbiology, S.S.E.S.A s Science College, Congress Nagar, Nagpur - 440 012, M.S., India.
International Journal of Current Microbiology and Applied Sciences 3 : 567-580
Abstract : Mangifera indica extract is used as a natural medicine. It is a cheaper and safe alternative source of drugs. Antibacterial activity of Mangifera indica bark extracts was determined using agar well diffusion method. It had significant antibacterial potency against the tested pathogens. In the present study the plant active components of M. indica bark were extracted using four different extraction solvents namely distilled water, ethanol, methanol and ethyl acetate. The phytochemical compounds such as steroids, cardiac glycosides, saponins and resins were revealed. It was demonstrated that more percentage of yields was observed for distilled water extract (27.34%) followed by ethanol (8.86%), methanol (2.28%) and least for ethyl acetate (1.15%). In the present study bark powder extract of M. indica was studied by using 100%, 50%, 25%, 12.5% and 6.25% dilution of four different solvents against the tested pathogens such as Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Klebsiella pneumoniae and Pseudomonas aeruginosa. The ethyl acetate extract (100%) was found to be most effective against Klebsiella pneumoniae (28 mm) followed by Bacillus subtilis (25 mm), Pseudomonas aeruginosa (22 mm), Staphylococcus aureus and Escherichia coli (20 mm each). Distilled water extract of Mangifera indica bark was not effective for the entire tested pathogens.