Early identification of male parent of mango seedlings by ISSR molecular markers.
Zhang Yu, Wang ChangJiang, Tang ZhiPeng, Zhou DaoJun
Author Affiliation: Agricultural College, Guangxi University, Nanning 530005, China.
Journal of Southern Agriculture 45 : 7-11
Abstract : Objective: Early identification and selection of parents of mango seedlings by ISSR molecular markers were conducted to provide references for improving mango breeding efficiency based on ISSR molecular markers. Method: Using ISSR marker technology, different primers were screened to identify male parent of 97-1 seedling. Result: According to the optimized ISSR reaction system, four specific primers were chosen from 18 primers (UBC-848, UBC-857, UBC-864 and UBC-868), which had many amplified bands, strong stability, high polymorphism and clear backgrounds. Jyhua mango, Sichuan red-skin mango and 97-1 seedlings were amplified using primers UBC-846, UBC-857, UBC-864 and UBC-868. The length of the amplified fragments was 100-2000 bp. The size of specific bands of Sichuan red-skin mango, Jyhua mango and 97-1 seedlings was 180 and 380 bp, 280 and 380 bp, 180 and 680 bp, and 680 and 770 bp. Conclusion: Through the identification system construction of mango seedling parents using ISSR molecular markers and primers UBC-846, UBC-857, UBC-864 and UBC-868, it was conclude that the male parent of 97-1 seedlings was Sichuan red-skin mango and 97-1 seedlings were the crosses between Jyhua mango and Sichuan red-skin mango.