Optimizing mangiferin extraction from Mangifera indica L. peel and analyzing its antibacterial activity.
Huang ChunPing, Luo Qun, Li Jie
Author Affiliation: College of Life Sciences, Sichuan Normal University, Chengdu 610066, China.
Journal of Southern Agriculture 45 : 1452-1456
Abstract : Objective: The extraction process of mangiferin from Mangifera indica L. (mango) peel was optimized and the antibacterial activity of extracts was detected to provide references for comprehensive utilization of mango and its by-products. Method: The effects of volume fraction of ethanol, liquid ratio, microwave power and extraction time on extraction of mangiferin from mango peel were researched using single factor and orthogonal experiments and the antibacterial activity of mangiferin crude extracts was detected by disc diffusion method of Kirby-Bauer (KB). Result: The order of factors influencing mangiferin extraction from mango peel was volume fraction of ethanol > extraction time > microwave power > liquid ratio. The content of mangiferin was 4.622 mg/g under the optimum conditions of 70% ethanol extraction, 600 W of microwave power, 1:10 liquid ratio and extract time of 15 min. Furthermore, the mangiferin crude extract had strong antibacterial activity to E. coli, S. aur and C. alb. In addition, minimum inhibitory concentration of the mangiferin crude extract to E. coli and S. aur was 1.250 mg/mL. and to C. alb was 0.625 mg/mL. Conclusion: Microwave-assisted extraction is an effective method for mangiferin extraction from mango peel and mangiferin had antibacterial activity in some extent.