Validation of a high performance liquid chromatography technique for determination of mangiferin content in Mangifera indica L. leaves.
Romero Díaz J. A., Nuva-Paz L., López M., Ferrada C., Carballo C.
Author Affiliation: Centro de Investigación y Desarrollo de Medicamentos (CIDEM), Ave 26 # 1605, La Habana, Cuba.
Revista Cubana de Plantas Medicinales 19 : 167-178
Abstract : Introduction: Mangiferin is a natural glycosylxanthone present in various parts of the tree Mangifera indica L. which is attributed a wide range of pharmacological properties. Objective: establish a method to determine the content of mangiferin in Mangifera Indica L. leaves by high performance liquid chromatography. Methods: leaves were dried and pulverized before quantification of their metabolite content. Separation was performed using a Luna RP-18 chromatographic column (5 µm) (250×4 mm) with a UV detector at 254n and a mobile phase A gradient composed of acetic acid 0.2%: acetonitrile (85:15), and a phase B mobile: acetonitrile and its quantification vs. a reference sample applying the external standard method. Results: the regression curve equation for mangiferin was Y=3.5561X+6536.55 (r=0.999). Recovery was 99.41% with a variation coefficient below 2%. Detection and quantification limits were 0.0009 and 0.001, respectively. No statistically significant differences were found between the determinations performed on different days by different analysts. Conclusion: the method is simple and reliable, and may be used for quality control and stability studies about Mangifera Indica L. leaves.