Biological characteristics of Cladosporium cladosporioides causing mango sooty blotch disease.
Yang YongLi, Pu JinJi, Zhang He, Wei YunXie, Li ZengPing, Liu XiaoMei
Author Affiliation: College of Environment and Plant Protection/Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources/Ministry of Education Hainan University, Hainan University, Haikou 570228, China.
Journal of Fruit Science 32 : 285-290
Abstract : Objective: To provide a scientific basis for studying the epidemiology of mango sooty blotch disease in field, a new disease on mango fruit, the biological characteristics of Cladosporium cladosporioides was tested. Methods: This paper studied the effect of different conditions on mycelium growth, sporulation and spore germination of C. cladosporioides. Results: For mycelial growth, the optimal medium was mango dextrose agar medium rich in organic nutrition. The optimum temperature was 20°C and the optimum pH value was 7-8. The effect of light was not significant. Lactose or nitrogen source glycine was respectively the optimum carbon or nitrogen source. Lethal temperature of mycelium was 50°C for 10 min. For sporulation, the optimum temperature was 25°C and the optimum pH value was 6. Alternation of light and dark, maltose and L-acystine were in favour of sporulation. For spore germination, the optimum temperature was 25°C, the optimum pH value was 7, and the optimum RH was liquid water. Organic carbon source arabinose or nitrogen source L-asparaginate were in favor of spore germination. Lethal temperature of spore was 50°C for 10 min. Conclusion: It showed that the environment of 20-25°C, pH 6-8 with high humidity, and medium rich in organic nutrition were favorite to C. cladosporioides.