Use of Plackett-Burman design for rapid screening of diverse raw pectin sources for cold-active polygalacturonase and amylase production by Geotrichum sp.
Divya K., Padma P. N.
Author Affiliation: Bhavan's Vivekananda College of Science, Humanities and Commerce, Secunderabad - 94, India.
International Journal of Current Microbiology and Applied Sciences 4 : 821-827
Abstract : Cold active polygalacturonases and amylases play significant role in extraction and clarification of fruit juices at industrial level. An optimized production medium with low cost substrates would be very useful for commercial production of these enzymes. The present study was done to screen low cost pectin and starch substrates for cold active enzymes production. The different pectin and starch sources screened were fruit and vegetables peels like citrus, pineapple, apple, banana, mango, guava, carrot, beetroot, bottle gourd, ridge gourd and potato. For efficient screening of the best sources a statistical design like Plackett-Burman was used as in this design n variables can be studied in just n-1 experiments only. A twelve experimental design Plackett-Burman was used as the best sources can be shortlisted in consideration with their interactive effects. The pectinolytic and amylolytic yeast isolate was identified as Geotrichum sps and was used for the present study. Cold-active pectinase and amylase enzyme activity was assayed by dinitrosalicylic acid (DNS) method. The flasks were incubated at room temperature (20-25°C) and cold active enzyme activity was tested at 5°C. The best pectin sources selected for efficient cold-active pectinase production were banana and bottle guard and for amylase mango and guava peels. Thus, these studies indicate that low cost raw sources can be used for optimization of production media for commercial production of cold-active polygalacturonase and amylase producer which could have better application in fruit juice clarification studies.