Cloning and expression analysis of chalcone synthase gene (CHS1) from mango.
Mei ZhiDong, Zhang He, Liu XiaoMei, Huang JianFeng, Pu JinJi
Author Affiliation: College of Environment and Plant Protection, Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, Ministry of Education Hainan University, Hainan University, Haikou 570228, Hainan, China.
Journal of Fruit Science 32 : 1077-1084
Abstract : Objective: The aims of this study was to clone the chalcone synthase (CHS) gene and analyze its function, and reveal its expression in different tissues of mango. Methods: Total DNA and RNA from Mango were used as template to conduct PCR amplification and a target fragment in the amplification was cloned to vector pMD18-T easily and then sequenced. The structure of the protein (CHS) was analyzed by bioinformatics. The expression analysis was finished by Semi-quantitative PCR. Results: Sequence analysis showed that the cDNA of CHS was 1 173 bp in length and contained two exons and one intron. The phylogenetic analyze based on the cDNA sequences showed that CHS shares high homology with the CHS1 from Mangifera indica. Semi-quantitative analysis showed that the CHS1 gene can be detected in all Mango tissues, but the gene expression levels in leaves and flowers were higher than those in other tissues. Conclusion: In this study, the CHS1 gene was cloned and analyzed for the expression in different tissues, which has laid the foundation for increasing the expression of CHS1.