Somatic embryos from cultured ovules of polyembryonic Mangifera indica L.
Litz R. E., Knight R. L., Gazit S.
Author Affiliation: Florida Univ. Agricultural Research & Education Center, Inst. of Food & Agricultural Sciences, Homestead, FL 33031, USA.
Plant Cell Reports 1 : 264-266
Abstract : Ovules removed from two-month-old fruits of nine naturally polyembryonic cultivars and one monoembryonic cultivar were cultured on modified Murashige and Skoog medium. The nucellus was removed from the cultured ovules between one and three weeks after culture and transferred onto the same medium. Somatic embryogenesis occurred from the nucellus of five of the naturally polyembryonic cultivars after 1-2 months in culture. The embryogenic response was cultivar dependent and appeared to be related to the degree of polyembryony in the ovules at the time of culture. Optimum somatic embryogenesis occurred in medium containing 20% coconut water.