In vitro somatic embryogenesis from nucellar callus of monoembryonic mango.
Litz R. E.
Author Affiliation: Univ. Fla. Inst. Food & Agric. Sci., Tropical Res. & Educ. Cent., Homestead, FL 33031, USA.
HortScience 19 : 715-717
Abstract : Calluses were induced from nucellar explants excised from fertilized ovules of immature (4.0-4.8 cm long) monoembryonic fruitlets of 9 Mangifera indica cultivars. Somatic embryogenesis occurred from callus on modified Murashige & Skoog medium, and following subculture to a medium lacking growth regulators. The highest percentages for both callus formation from explant cultures and embryoid formation from callus cultures were recorded for Irwin (60% and 40%, respectively) followed by Ruby (52% and 23.1%) and Tommy Atkins (36% and 11.1%).