Polyamines in adventitious and somatic embryogenesis in mango (Mangifera indica L.).
Litz R. E., Schaffer B.
Author Affiliation: Univ. Florida, IFAS, Trop. Res. & Education Center, Homestead, FL 33031, USA.
Journal of Plant Physiology 128 : 251-258
Abstract : Polyamine contents were measured in nucelli, embryogenic (E) and non-embryogenic (NE) nucellar calluses, adventitious nucellar embryos, nucellar somatic embryos and zygotic embryos of several monoembryonic (M) and polyembryonic (P) cultivars. In vitro conditions caused an up to 50-fold increase in putrescine concentration (nucellar callus vs. nucellus), the increase being greater in NE than E callus and greater in Keitt (M) than Parris (P). Polyamine concentrations in general were higher in NE than E callus and higher in callus from M than from P cultivars. Nucellar callus from the P cultivars Parris and Peach was more E than that from the M cultivars Irwin and Tommy Atkins; Keitt was comparable to the P cultivars. Polyamines added to the culture medium generally had no effect on callus initiation or somatic embryogenesis, except that spermidine suppressed callus formation in Keitt. Callus induction, but not somatic embryogenesis, was enhanced by added 2,4-D.