High -performance liquid chromatographic determination of D-arabino-hexos-2-ulose (D-glucosone) in irradiated sugar solutions: application of the method to irradiated mango.
Drijver L. deu, Holzapfel C. W., Linde H. J. van der
Author Affiliation: Chem. Dep., Atomic Energy Corp., Private Bag X256, Pretoria 0001, South Africa.
Journal of Agricultural and Food Chemistry 34 : 758-762
Abstract : A strongly basic anion exchange HPLC column coupled with an amperometric detector was used for the direct detection of the mutagenic compound D-glucosone in ? irradiated sugar solutions. The method was applied to an irradiated model fruit system and to mangoes (cv. Kent) which were irradiated to doses of 0.75 (the commercial dose recommended for the preservation of subtropical fruits), 1.5, 5 and 20 kGy. Although D-glucosone was detected in the model system, it was not detected in whole fruits even when they were irradiated to 20 kGy. The results indicate that components of the fruit provide considerable protection against the radiolytic formation of D-glucosone and that the model system should not be used without qualification to simulate the radiolytic behaviour of real fruit.