Elucidation of some in vitro exo-pectolytic enzymes by mango anthracnosis fungus Colletotrichum gloeosporioides Penz.
Prakash P., Manoharachary C., Bochow H.
Author Affiliation: Mycol. Pl. Path. Lab., Bot. Dep., Osmania Univ., Hyderabad, India.
Archiv für Phytopathologie und Pflanzenschutz 25 : 197-199
Abstract : During a survey of market diseases of mango fruits around Hyderabad, India, c. 20-30% of the fruits showed rotting symptoms. The fungus most frequently isolated was C. gloeosporioides [Glomerella cingulata]. In vitro production of exo-polygalacturonase (Exo-PG), exo-polymethylgalacturonase (Exo-PMG), exo-pectic acid lyase and exo-pectin lyase activities was assayed on 6 different media. In all cases, a linear increase in enzymic activity was found from the 5th to 10th days of incubation, after which activities decreased. Exo-PG and Exo-PMG were no longer produced by the 15th day. The role of these enzymes in pathogenesis is discussed.