Optimizing somatic embryo production in mango.
DeWald S. G., Litz R. E., Moore G. A.
Author Affiliation: Department of Fruit Crops, University of Florida, 1137 Fifield Hall, Gainesville, FL 32611, USA.
Journal of the American Society for Horticultural Science 114 : 712-716
Abstract : Growth of embryogenic cultures of Mangifera indica in suspension was more efficient than on solid medium; however, subculture onto solid medium was essential for high-frequency production of morphologically normal somatic embryos, and Gelrite was more effective in this respect than Difco Bacto-agar. Modified B-5 basal medium was better for maintenance of cultures and for production of morphologically normal somatic embryos than either MS or woody plant medium. Sucrose concentrations of 5-6% were optimal for somatic embryo production, and also increased the frequency of recovery of normally differentiated early heart-shaped somatic embryos. Coconut water (20%, v/v) enhanced somatic embryo production by 18%. Other complex organic supplements alone or in combination with coconut water were either ineffective (casein hydrolysate) or highly inhibitory (yeast extract) in comparison with basal medium alone.