Maturation and germination of mango somatic embryos.
DeWald S. G., Litz R. E., Moore G. A.
Author Affiliation: Department of Fruit Crops, 1137 Fifield Hall, Gainesville, FL 32612, USA.
Journal of the American Society for Horticultural Science 114 : 837-841
Abstract : Maturation and germination of mango (Mangifera indica) somatic embryos was achieved by sequential transfer of heart-shaped somatic embryos on a series of media based on the B5 formulation. Precocious germination and other developmental abnormalities were controlled by incorporating 3 µM abscisic acid (ABA) and 6% sucrose in the medium. Other factors, including the substitution of Gelrite (0.175%) for agar, the use of solid rather than liquid medium and culture of somatic embryos in darkness until physiological maturity, also affected normal growth and development. Coconut water (20%) was also essential for somatic embryogenesis. Germinated somatic embryos were successfully established in planting medium.