Cell wall degrading enzyme production by Colletotrichum gloeosporioides the cause of mango anthracnose.
Kanakaratne N. S., Adikaram N. K. B.
Author Affiliation: Department of Botany, University of Peradeniya, Peradeniya, Sri Lanka.
Ceylon Journal of Science, Biological Sciences 21 : 41-54
Abstract : C. gloeosporioides [Glomerella cingulata] produced pectolytic, proteolytic and cellulolytic enzymes in culture media incorporated with water-insoluble components from unripe or ripe mango fruit. Pectin, protein and cellulose constituents of both ripe and unripe fruits appeared equally accessible to the pathogen. Culture filtrates of G. cingulata grown on components from unripe and ripe fruit and extract of autoclaved unripe fruit tissue rotted by the fungus contained the pectic enzymes exopolygalacturonase (PG) and exo-pectate lyase (PL), whereas the extract from ripe fruit tissue rotted by the fungus contained exo-PG and endo-PG. The pH optima of polygalacturonases (exo- and endo-) and pectin lyases in all these enzyme preparations were 5.5 and 8.5, respectively. These results are discussed and compared with those obtained by other workers, using G. cingulata and other pathogens.