Physiological and biochemical factors associated with fruit bud differentiation in Alphonso mango. III-Total protein and RNA.
Patil P. B., Rao M. M., Janardhan K. V., Srinivasan C. N., Nalawadi U. G.
Author Affiliation: College of Agriculture, Dharwad 580?005, India.
Karnataka Journal of Agricultural Sciences 3 : 208-212
Abstract : The RNA and total protein contents of Alphonso mango buds were analysed at half-month intervals during fruit bud differentiation (FBD, a total period of about 2.5 months) and at 4-h intervals on each sampling date. At all stages of differentiation, the mean total RNA content peaked at 08.00 and 20.00 h and total protein content decreased at the same times. At the onset of FBD, mean RNA content decreased (from 3.3 to 2.2 mg/g FW) and mean protein content increased (from 108.7 to 147.5 mg/g FW). Both then increased during differentiation. Protein content decreased during the post-differentiation stage in vegetative scar buds and in undifferentiated buds. RNA and protein contents were higher in differentiated buds than in undifferentiated and scar buds (during post-FBD, mean RNA contents were 3.4, 2.7 and 2.9 mg/g FW, respectively, and mean protein contents were 217.6, 149.4 and 172.4 mg/g FW, respectively). Stained sections showed that RNA content was highest at the apex of the floral bud.