Effect of growth regulator and media on in vitro shoot tip culture of different cultivars of mango (Mangifera indica L.) rootstocks.
Yang Z. H., Lüdders P.
Author Affiliation: Fruit Science Section, Technical University of Berlin, Albrecht-Thaer-Weg 3, 1000 Berlin 33, Germany.
: 240-247
Abstract : Various plant growth regulators and proprietary mixtures were used to supplement the medium for in vitro shoot tip propagation of mango. Shoot growth was better in G medium [see Yang, Z. H.; Hu, N. Y.; Lu, G. M. Acta Univ. Sept. Occ. Agri. (1984) 1, 13-19] supplemented with the combination of IBA (0.5 mg/litre), BA, zeatin and IAA (each at 1.0 mg/litre), 2iP [isopentenyladenine] (2 mg/litre), casein hydrolysate and glutamine (300 mg/litre) and 3% sucrose, than in B-5 basal medium or woody plant medium. The addition of GA3 (5-10 mg/litre) to the medium, did not improve shoot growth. Shoot tip initiation and elongation were greater in cv. Turpentine than in the cultivars 13-1 and Gomera. Shoot production was greatest with explants taken during May and June.