A simple ion-exchange chromatographic determination of non-volatile organic acids in some Spanish exotic fruits.
Cano M. P., Torija E., Marín M. A., Cámara M.
Author Affiliation: Department of Freezing of Vegetable Products, Instituto del Frío, Spanish Council for Scientific Research, Ciudad Universitaria, E-28040 Madrid, Spain.
Zeitschrift für Lebensmittel-Untersuchung und -Forschung 199 : 214-218
Abstract : The production of certain exotic fruits in Spain has continued to increase in recent years, banana being the most popular, followed by pineapple and kiwifruit. HPLC was used to analyse fruit samples of the kiwifruit cultivars Hayward, Bruno, Monty and Abbot, mango cultivars Smith and Lippens, pawpaw cv. Solo, pineapple cv. Smooth Cayenne and banana cv. Enana prepared by methanol extraction, followed by a clean-up with a C18 cartridge. Separation of the different acids was accomplished using an ion-exchange column and detection was monitored at 214 nm. Reproducibility of the minor organic acids was quite acceptable and the procedure described offers a rapid analytical alternative to gas chromatography. Oxalic, oxoglutaric, citric, galacturonic, tartaric, ascorbic, L-malic, quinic, succinic, fumaric and D-malic acids could be quantified by this method.