Antigiardiasic activity of 14 plant extracts in vitro.
Ponce-Macotela M., Navarro-Alegría I., Martínez-Gordillo M. N., Alvarez-Chacón R.
Author Affiliation: Laboratorio de Investigación en Parasitología, Instituto Nacional de Pediatría, Insurgentes Sur No. 3700-C, 04530 Mexico.
Revista de Investigación Clínica 46 : 343-347
Abstract : The viability of Giardia duodenalis trophozoites following incubation with one of 14 plant extracts was investigated using MTT (3-[4,5-dimethylthiazol-2-il]-2, 5-diphenyl tetrazolium bromide), reduced to MTT-formazan by the activity of live trophozoites. Both negative (trophozoites without extracts) and positive (trophozoites incubated with tinidazole) controls were included. The percentages of mortality were calculated by spectrophotometry of the reduced MTT at 570 nM: Justicia spicigera 91%; Lippia berlandieri 90%; Psidium guajava 87%; positive control incubated with tinidazole 79%; Punica granatum 78%; Mangifera indica 77%; Plantago major 76%; Cupressus sempervirens 73%; Castela tormentosa 70%; Hematoxilon campechanum [Haematoxylum campechianum] 67%; Persea americana 23%; Capsicum annuum 21%; Oryza sativa 5%; Prosopis juliflora 0%; Rhizophora mangle 0%.