Plant regeneration of Mangifera indica using liquid shaker culture to reduce phenolic exudation.
Raghuvanshi S. S., Srivastava A.
Author Affiliation: In Vitro Culture and Plant Genetics Unit, Department of Botany, University of Lucknow, Lucknow 226007, India.
Plant Cell, Tissue and Organ Culture 41 : 83-85
Abstract : The exudation of phenolics from the cut ends of mango explants greatly hinders their regenerative ability in any in vitro growth medium. Pretreatment of explants using liquid shaker culture helped overcome this problem. Explants kept in liquid MS medium supplemented with 1% polyvinylpyrolidone [polyvidone] in 250 ml conical flasks on an automated shaker at 75 r.p.m. produced shoots when inoculated on gelled MS medium supplemented with different concentrations of growth regulators.