Peroxisomal thiolase mRNA is induced during mango fruit ripening.
Bojorquez G., Gómez-Lim M. A.
Author Affiliation: CINVESTAV-Unidad Irapuato, Apartado Postal 629, Irapuato, Guanajuato, Mexico.
Plant Molecular Biology 28 : 811-820
Abstract : By differential screening of a cDNA library prepared from ripe mango (Mangifera indica cv. Manila) fruit with RNA poly(A)+ from unripe and ripe mesocarp, a number of cDNAs expressed only in ripe fruit have been isolated. This paper reports the characterization of one such cDNA (pTHMF1) from M. indica which codes for a protein highly homologous to cucumber, rat and human peroxisomal thiolase (EC 2.3.1.16, acetyl-CoA C-acetyltransferase), the catalyst for the last step in the ?-oxidation pathway. The cDNA for the peroxisomal mango thiolase is 1305 bp in length and codes for a protein of 432 amino acids with a predicted molecular mass of 45?532 Da. Mango thiolase is highly homologous to cucumber thiolase (80%), the only other plant thiolase whose cloning has been reported, and to rat and human thiolases (both 55%). Northern analysis indicated that THMF1 is up-regulated during fruit ripening. A similar pattern of expression was detected in tomato fruit. Wounding and pathogen infection did not appear to affect THMF1 expression. The possible involvement of thiolase in fatty acid metabolism during fruit ripening is discussed. Nucleotide sequence data have been submitted to the EMBL/GenBank/DDBJ databases under the accession number X75329.