Production of monoclonal antibodies against Xanthomonas campestris pv. mangiferaeindicae and their use to investigate differences in virulence.
Sanders G. M., Verschoor J. A., Wyngaard S. van, Korsten L., Kotzé J. M.
Author Affiliation: Department of Microbiology, University of Pretoria, Pretoria, South Africa.
Journal of Applied Bacteriology 77 : 509-518
Abstract : Four X. c. pv. mangiferaeindicae isolates from mango black spot lesions were grouped according to differences in virulence and used to raise monoclonal antibodies (MAbs). Two immunization approaches were followed. In the first, 4 groups of mice were immunized, each with a different isolate and the spleens from each group homogenized together for cell fusion. The second approach entailed immunization of a single group of mice with bacteria pooled from all 4 isolates. The resultant MAbs were characterized with regard to the antigen binding specificity and antibody class. A relationship between MAb binding specificity and virulence of the bacteria was shown by Western blot analysis.