Electroantennographic and coupled gas chromatographic-electroantennographic responses of the Mediterranean fruit fly, Ceratitis capitata, to male-produced volatiles and mango odor.
Cossé A. A., Todd J. L., Millar J. G., Martínez L. A., Baker T. C.
Author Affiliation: Department of Entomology, University of California, Riverside, California 92521, USA.
Journal of Chemical Ecology 21 : 1823-1836
Abstract : Five compounds from the headspace of calling males of Ceratitis capitata and 3 compounds from the headspace of ripe mango (Mangifera indica) were identified using coupled GC-electroantennographic, GC-MS and electroantennographic (EAG) assays of standards. The male-produced volatiles eliciting responses from female antennae were ethyl-(E)-3-octenoate, geranyl acetate, (E,E)-?-farnesene, linalool and indole. An EAG dose-response test of linalool enantiomers and indole with the antennae of C. capitata showed relatively strong EAG activities, but no significant difference between (R)-(-)-linalool and (S)-(+)-linalool. The 3 mango volatiles were identified as (1S)-(-)-?-pinene, ethyl octanoate and ?-caryophyllene. In addition, a strong antennal response was recorded from a contaminant, ?-copaene, present in a commercial sample of ?-caryophyllene. The EAG response amplitudes from both male and female antennae to the above 3 mango volatiles were significantly greater than to a hexanol control. For both male and female antennae, the greatest EAG responses were elicited by ?-caryophyllene followed by ethyl octanoate. The mean EAG responses of female antennae to ?-caryophyllene and (1S)-(-)-?-pinene were significantly greater than those of male antennae.