Isolation and characterization of soluble polysaccharides and insoluble cell wall material of the pulp from four mango (Mangifera indica L.) cultivars.
Ollé D., Lozano Y. F., Brillouet J. M.
Author Affiliation: Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Département FLHOR, 2477 Avenue du Val de Montferrand, B.P. 5035, 34032 Montpellier Cedex 1, France.
Journal of Agricultural and Food Chemistry 44 : 2658-2662
Abstract : Mature green fruits from monoembryonic (Amélie and Palmer) and polyembryonic (M'Bingue and Tête de Chat) mango cultivars obtained from the experimental orchard at Korhogo, Côte d'Ivoire, were initiated to ripen with ethylene (10 ppm, 24 h) and then left to reach full ripeness (6 days). After elimination of skin and kernel, pulp was washed with HEPES buffer and centrifuged. Soluble polysaccharides were obtained from the supernatant by precipitation with ethanol and freeze-drying. Cell wall material (CWM) was isolated from the pellet by the buffered phenol procedure and further enzymatically destarched. Soluble polysaccharides (~0.5-0.8% pulp FW) were essentially highly esterified pectic substances (uronic acids content ~50-60%; degree of methyl esterification ~89-97%) and their MW were higher in the polyembryonic cultivars. CWM, ~1% pulp fresh weight, was mainly cellulose (~20%) and highly esterified pectic substances (uronic acids ~13-24%; degree of esterification ~63-73%). Hemicellulosic glucans were more abundant in the monoembryonic (~9%) than in the polyembryonic (~4%) cultivars.