HPLC and mass spectrometric analysis of carotenoids from mango.
Mercadante A. Z., Rodriguez-Amaya D. B., Britton G.
Author Affiliation: Departamento de Ciência de Alimentos, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas, C.P. 6121, 13081-970 Campinas, Brazil.
Journal of Agricultural and Food Chemistry 45 : 120-123
Abstract : ?-Carotene (all-trans), ?-cryptoxanthin (all-trans and cis), zeaxanthin (all-trans), luteoxanthin isomers, violaxanthin (all-trans and cis) and neoxanthin (all-trans and cis) were identified in mango by means of HPLC data (retention time, co-injection, and chemical reactions). Mass spectrometry (molecular ion, fragmentation pattern, m/z relative intensity) confirmed the identity of these carotenoids (not the geometric form); additionally, lutein and mutatochrome were detected when a large amount of sample was utilized. The quantitative composition was determined by HPLC, Sudan I being used as internal standard. all-trans-Violaxanthin (21.1±2.9 µg/g), all-trans-?-carotene (15.1±1.5 µg/g) and a cis-violaxanthin (10.1±0.2 µg/g), tentatively identified as 9-cis, were the principal carotenoids.