Trials of using in vitro techniques for vegetative propagation of mangos.
Reuveni O., Golubowicz S.
Author Affiliation: The Volcani Center, ARO, PO Box 6, Bet-Dagan, Israel.
: 496-504
Abstract : Experiments with small internodes (for multishoot propagation via organogenesis) and excised nucelli established in culture to obtain embryogenic mango cultures are reported. Cultures were incubated in the dark at 28°C. When small internodes were cultured, browning of the cultures and endophytic contamination were the 2 main factors which limited establishment and proliferation. Different media and supplements including activated charcoal and specific antibiotics did not improve the efficiency of shoot formation in established cultures. Ovules were isolated from 7 cultivars at 5 different stages of flowering and fruiting from the end of May to the end of June 1990. Survival rates of non-contaminated explants, and the rate of embryogenic cultures obtained, were determined by the age of the fruitlets. Two media were found to be advantageous and were composed of (in mg/l): (1) macroelements of MS (Murashige and Skoog) medium (1/2 strength), thiamine HCl (0.5), glutamine (400), ascorbic acid (100) and 6% sucrose; and (2) macroelements of WPM (Woody Plant Medium), thiamine HCl (1.0) and 3% sucrose. The common supplements added to both media were 2,4-D (50), activated charcoal (0.25%) and agar (0.65%). Highly proliferating embryogenic cultures were obtained from some cultivars. Some initially non-embryogenic cultures formed embryos at a later stage. Once these embryogenic cultures were formed they continued to proliferate as embryogenic cultures for several years.