Non-resorcinol antifungal compounds in mango peel (Mangifera indica L.).
Supyen D., Chairangai N., Sardsud V.
Author Affiliation: Chemistry Department, Faculty of Science, Chiang Mai University, Chiang Mai 50002, Thailand.
ACIAR Proceedings Series : 115-120
Abstract : An antifungal compound was isolated from the peel of unripe mango (Chok-anan). Concentrated ethanol-dichloromethane extract of the peel when bioassayed on thin-layer chromatographic plates (TLC) with conidia of Cladosporium cladosporioides produced an inhibition area at Rf~0.15. Concentrations of the antifungal compound above 20 µg/µl inhibited growth of the fungal colonies. The crude extract was partitioned on a silica gel column and the antifungal compound was separated by hexane:ethyl-acetate (50:50 v/v) elution and purified again by preparative TLC technique. Spectroscopic data indicate that these antifungal compounds consisted of 2 parts: the major component was di-2-ethylhexyl phthalate, the other was a mixture of various alkyl phthalate compounds. However, the same compounds brought from the BDH company could not inhibit the growth of the fungus. The same experiments were conducted with other parts of mangoes such as leaf, inner layer (mesocarp) and seed. The substances obtained from all these parts were not found to inhibit the fungal colony expansion.