Geographic differentiation and embryo type identification in Mangifera indica L. cultivars using RAPD markers.
López-Valenzuela J. A., Martínez O., Paredes-López O.
Author Affiliation: Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Sinaloa, Apdo. Postal 20145, 80000 Culiacan Sin, Mexico.
HortScience 32 : 1105-1108
Abstract : Some 15 mango cultivars collected from Mexico, Phillipines, Florida and Réunion, were examined using RAPD markers with decamer primers of arbitrary sequence. Thirteen of the 40 primers screened were informative and 109 amplified DNA bands were selected as RAPD markers. Specific RAPD markers for some mango cultivars were identified. Cluster analysis based on the RAPD markers produced a dendrogram of the genetic relatedness of the 15 mango cultivars. Manila and Carabao were the most similar, which is in good agreement with their putative pedigrees. The 4 major bifurcations in the dendrogram clearly separated the genotypes into 4 groups according to their geographic origin. Bulk segregant analysis of polyembryonic and monoembryonic cultivars detected a specific RAPD marker for polyembryony. These markers may facilitate the management of mango germplasm for breeding purposes.