Effects of 1-aminocyclopropane-1-carboxylic acid, aminoethoxyvinylglycine, methylglyoxal bis-(guanylhydrazone) and dicyclohexylammonium sulfate on induction of embryogenic competence of mango nucellar explants.
Litz R. E., Yurgalevitch C.
Author Affiliation: University of Florida, Tropical Research and Education Center, 18905 S.W. 280 Street, Homestead, FL 33031-3314, USA.
Plant Cell, Tissue and Organ Culture 51 : 171-176
Abstract : Effects of aminoethoxyvinylglycine (AVG),1-aminocyclopropane-1-carboxylic acid (ACC), dicycyclohexylammonium sulfate [dicyclohexamine] (DCHA) and methylglyoxal bis(guanylhydrazone) (MGBG) on induction of embryogenic competence from nucellus of Mangifera indica cultivars Tutehau (TT; polyembryonic) and Tommy Atkins (TA; monoembryonic), were compared. Induction of embryogenic competence in explanted nucellus of TA was more sensitive to AVG and DCHA than TT. MGBG had no effect on induction of embryogenic competence of either genotype, and ACC suppressed somatic embryogenesis in both cultivars. Ethylene biosynthesis was greater in TA explants. Reducing ethylene biosynthesis by addition of AVG marginally increased the number of embryogenic cultures of TT, but completely inhibited somatic embryogenesis in TA cultures. Ethylene biosynthesis was stimulated by ACC in non-embryogenic cultures of both genotypes and in TA embryogenic cultures, in which ethylene biosynthesis was ~7 times greater than in TT embryogenic cultures. The greater sensitivity of TA to the ethylene antagonist, AVG, and DCHA, an inhibitor of spermidine synthase, suggests that the absence of nucellar embryony in vivo in TA may be due to its greater sensitivity to disturbance of ethylene biosynthesis and spermidine synthesis.