Changes in gene expression during mango fruit ripening.
Chaimanee P., Suntornwat O., Lerrtwikool N., Bungaruang L.
Author Affiliation: Department of Chemistry, Faculty of Science, Silpakorn University, Nakorn Pathom 73000, Thailand.
Journal of Biochemistry, Molecular Biology and Biophysics 3 : 75-79
Abstract : Changes in gene expression during ripening were studied in mango (Mangifera indica) fruits. Total soluble proteins were extracted at various stages of ripening, and analysed by one-and two-dimensional gel electrophoresis. A considerable increase of 64, 42, 39 and 26 kDa polypeptides was observed. Similarly, total RNA and poly(A)+ mRNA were isolated and subjected to in vitro translation. The amount of total RNA increased during the ripening process, especially from the unripe to ripe stage. Analysis of translated products by SDS gel electrophoresis and autoradiography showed the accumulation of 67, 61 and 48 kDa polypeptides. The results indicated that the ripening process in mango is associated with the expression of specific genes.