Identification of mango cultivars of Thailand and evaluation of their genetic variation using the amplified fragments by simple sequence repeat-(SSR-) anchored primers.
Eiadthong W., Yonemori K., Sugiura A., Utsunomiya N., Subhadrabandhu S.
Author Affiliation: Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
Scientia Horticulturae 82 : 57-66
Abstract : Twenty-two mango (Mangifera indica) cultivars, including 13 Thai, 4 Florida, 3 Indian, one Indonesian and one Philippine cultivar, were examined for genetic variation using SSR-anchored primers of 15-18 oligonucleotides. Of the 40 primers screened, 7 gave reproducible, polymorphic DNA amplification patterns, and were selected to construct a DNA fingerprinting table to distinguish the mango genotypes. The number of bands generated ranged from 8 to 21 per primer. Banding patterns obtained using these 7 primers allowed each cultivar to be distinguished from the others, indicating that polymerase chain reaction using SSR-anchored primers was an efficient method for cultivar identification. Similarity coefficients were calculated based on 56 selected bands and UPGMA [unweighted pair-group method with arithmetic means] cluster analysis was performed. Thai mango cultivars Nang Klangwan and Nong Saeng were very far distant of the genetic relationship from the other cultivars. The remaining 11 Thai cultivars were classified into 3 groups based on the dendrogram. Seven cultivars were placed in the same group as two Florida cultivars (Brooks and Edward), Philippine cv. Carabao and Indonesian cv. Arumanis. Four other Thai cultivars were scattered into two groups; each group contained Indian cultivars. Polyembryonic and monoembryonic seed races could not be distinguished clearly.