Exo-polygalacturonase from ripe mango (Mangifera indica Linn cv. Nam Dok Mai).
Chaimanee P., Lertwikoon N., Bungaruang L., Suntornwat O.
Author Affiliation: Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom, 73000, Thailand.
: 171-175
Abstract : Polygalacturonase (PG) is the most studied cell wall hydrolase. Both exo-PG (EC 3.2 1.67) and endo-PG (EC 3.2.1.15) were detected during the ripening process in mango. The increase in exo-PG was highly correlated with ripening whereas endo-PG activity was constant. Exo-PG was extracted from ripe mango mesocarp tissue. The enzyme has the relative molecular weight of 66?000 and 58?000 as detected by SDS-polyacrylamide gel electrophoresis. In Northern blot analysis, there was a barely detectable signal with the PG probe.