Transient and stable transformation in mango by particle bombardment.
Cruz-Hernandez A., Town L., Cavallaro A., Botella J. R.
Author Affiliation: Plant Genetic Engineering Laboratory, Department of Botany, University of Queensland, Brisbane, Qld 4072, Australia.
Acta Horticulturae : 237-242
Abstract : Proembryogenic masses of polyembryonic mango (Mangifera indica) cultivars Carabao and Kensington Pride were used as a target tissue for particle bombardment. The ?-glucuronidase (gus A) was used as reporter gene. The parameters associated with particle bombardment were optimized using transient expression assays of the ?-glucuronidase gene in proembryogenic masses. Osmotic treatment and particle acceleration pressure had a major effect on GUS transitory expression. Under optimal conditions, more than 1000 GUS foci were observed per microgram of bombarded DNA. Stable transformation assays of proembryogenic tissue were initiated using the green fluorescent protein as reporter gene. The implications of the system for transformation and regeneration of embryogenic tissue are discussed.