Amplified fragment length polymorphism analysis for studying genetic relationships among Mangifera species in Thailand.
Eiadthong W., Yonemori K., Kanzaki S., Sugiura A., Utsunomiya N., Subhadrabandhu S.
Author Affiliation: Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Journal of the American Society for Horticultural Science 125 : 160-164
Abstract : The phylogenetic relationships among 14 Mangifera species including 3 economically important species, i.e., common mango (M. indica), horse mango (M. foetida) and kwini (M. odorata), were analysed by comparing 217 amplified fragment length polymorphism (AFLP) markers. The unweighted pair grouping method using arithmetic averages (UPGMA) and neighbour-joining (NJ) method were used and 2 outgroup taxa, cashew nut (Anacardium occidentale) and gandaria (Bouea macrophylla), were added to both analyses. The common mango was closely related to banana mango (M. sylvatica), M. laurina, and M. oblongifolia. Intraspecific variation among 7 cultivars of common mango was much smaller than interspecific variation and these cultivars were classified into one M. indica group using both methods. M. macrocarpa, M. foetida, and M. odorata were also related to M. indica in both UPGMA and NJ trees, although these 3 species are classified into a different subgenus (subgenus Limus) from the subgenus Mangifera to which M. indica belongs. Also, in both UPGMA and NJ trees, M. gedebe [M. gedebi] and M. griffithii were placed in distant positions among the Mangifera species tested, indicating these 2 species are related distantly to M. indica. The AFLP technique was confirmed to be useful for phylogenetic analysis.