Somatic embryogenesis and plantlet regeneration in Amrapali and Chausa cultivars of mango (Mangifera indica L.).
Hussain Ara, Uma Jaiswal, Jaiswal V. S.
Author Affiliation: Laboratory of Morphogenesis, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221 005, India.
Current Science 78 : 164-169
Abstract : Somatic embryogenesis has been obtained from nucellus of two monoembryonic Indian mango cultivars Amrapali and Chausa. Among the four auxins (IAA, IBA, NAA & 2,4-D) tested, only 2,4-D stimulated callus initiation and induction of proembryogenic callus in cultured bisected ovules containing nucellus minus zygotic embryos. The proembryogenic calli produced up to 130 somatic embryos when transferred to 2,4-D-free medium. The presence of 2,4-D in the medium inhibited progression of development of somatic embryos. The best medium for the production, development and maturation of somatic embryos was the modified M4E medium which contained full-strength B5 macrosalts, MS microsalts, MS iron-EDTA and MS organics along with 400 mg/l L-glutamine, 6% (w/v) sucrose and 0.8% (w/v) agar. The mature somatic embryos gave rise to plantlets in liquid medium containing half-strength B5 macrosalts and 1.0 mg/l GA3. The in vitro raised plantlets of Amrapali cultivar have been successfully transplanted in earthen pots containing garden soil, but those of Chausa failed to survive in the garden soil but have been established in pots containing sand and soil (3:1) mixture.