Identification of an ETR 1-homologue from mango fruit expressing during fruit ripening and wounding.
Gutierrez Martínez P., López Gómez R., Gómez-Lim M. A.
Author Affiliation: CINVESTAV-Irapuato, Km 9.6 Carretera Irapuato-León, Apartado Postal 629, Irapuato GTO, Mexico.
Journal of Plant Physiology 158 : 101-108
Abstract : We have isolated a mango (Mangifera indica) cDNA homologue of the ethylene receptor gene ETR-1, referred to as METR1, which codes for a polypeptide of 802 amino acids with a predicted molecular weight of 89 kDa. The amino acid sequence is highly homologous (over 80%) to ETRs from other fruits. Genomic Southern blot analysis indicates that two or more ETR homologues exist in mango. RNA blot analysis revealed that the level of METR1 mRNA in the mesocarp increased during fruit ripening. In addition, it was found that the METR1 mRNA increases transiently during wounding of the tissue. This is the first report of an ETR homologue showing an induction during fruit ripening and wounding.