Determination of plant secondary metabolites by HPLC - recent developments.
Schieber A., Hilt P., Marx M., Carle R.
Author Affiliation: Universität Hohenheim, Institut für Lebensmitteltechnologie, Fachgebiet Lebensmittel pflanzlicher Herkunft, Garbenstrasse 25, D-70599 Stuttgart, Germany.
: 425-428
Abstract : A new stationary phase with hydrophilic endcapping has been used for the determination of phenolic compounds in extracts of apples, pears, mango, red wine and grape pomace. Since excellent resolution, especially of flavonoids was obtained, the method has also been applied in authenticity control studies of apple juices and pear juices. Furthermore, an analytical system for the quantitative determination of all-trans-?-, all-trans-?-, 9-cis-?-, and 13-cis-?-carotene in carrot juices and ATBC drinks has been established. The use of this method has been extended to the simultaneous extraction and quantification of carotenes and tocopherols. Baseline separation was obtained for ?-, ?-, ?-, ?-tocopherol, and ?-tocopheryl acetate, while the resolution of carotene isomers was not adversely affected.