Analysis of genetic diversity of Indian mango cultivars using RAPD markers.
Karihaloo J. L., Dwivedi Y. K., Sunil Archak, Gaikwad A. B.
Author Affiliation: National Research Centre on DNA Fingerprinting, NBPGR, Pusa Campus, New Delhi - 110 012, India.
Journal of Horticultural Science and Biotechnology 78 : 285-289
Abstract : Random amplified polymorphic DNA analysis was carried out in 29 Indian mango cultivars comprising popular landraces and some advanced cultivars. PCR amplification with 24 primers generated 314 bands, 91.4% of which were polymorphic. Jaccard's similarity between pairs of cultivars ranged between 0.318 and 0.75 with a mean of 0.565. A UPGMA dendrogram showed the majority of the cultivars from northern and eastern regions of India clustering together and separate from southern and western cultivars. Analysis of molecular variance revealed that 94.7% of the genetic diversity in mango existed within regions. However, differences among regions were significant; northern and eastern regions formed one zone and western and southern regions formed another zone of mango diversity in India.