High performance liquid chromatographic determination of imazalil residue in agricultural products.
Su HsiuChin, Lin AYang
Author Affiliation: Southern District Laboratory, Bureau of Food and Drug Analysis, Department of Health, Executive Yuan 180 Zihyou 2nd Rd., Zuoying District, Kaohsiung City 813, Taiwan.
Journal of Food and Drug Analysis 11 : 296-301
Abstract : A simple, rapid and reproducible residue analytical method for imazalil in fruits and vegetables was developed. The method involved the use of an ion-exchange cartridge for sample clean up followed by high performance liquid chromatography with ultraviolet detector. Acetic ether was used for extraction. Bond Elut PRS and Bond Elut C18 were used for purification. The chromatography column used Inertsil ODS-3 and the mobile phase was created by acetonitrile-10 mM KH2PO3 solution (pH 2.5), in the ratio of 35:65. The detection limit is 0.01 ppm. Rape, cabbage, honeydew melon, cumquat (mandarin), mango and maize were each spiked with 0.05-3.0 ppm imazalil to be compared with the recovery study. The yield was 88.7-97.9% for rape, 72.5-87.4% for cabbage, 81.4-90.5% for honeydew melon, 81.9-87.2% for cumquat, 75.0-85.3% for mango, and 86.8-99.0% for maize. When the method was applied in analysing commercial fruits and vegetables, no imazalil residue was detected in 20 selected samples (purchased from supermarkets and grocery stores in Kaohsiung, Taiwan).