Direct somatic embryogenesis induced from cotyledons of mango immature zygotic embryos.
Xiao JieNing, Huang XueLin, Wu YongJie, Li XiaoJu, Zhou MingDe, Engelmann F.
Author Affiliation: The Key Laboratory of Gene Engineering of the Ministry of Education, Zhongshan University, Guangzhou, 510275, China.
In Vitro Cellular & Developmental Biology - Plant 40 : 196-199
Abstract : For the first time, regenerated plantlets were obtained from immature zygotic embryos of mango (Mangifera indica) through direct somatic embryogenesis. Pro-embryogenic mass (PEM)-like structures, which are differentiated as clusters of globular structures, were easily induced directly from the abaxial side of cotyledons from immature fruits, 2.0-3.5 cm diameter by a 2-week culture period on a modified Murashige and Skoog medium with 5 mg litre-1 (25 µM) indole-3-butyric acid (IBA). Conversion of somatic embryos into plantlets was achieved after 4 weeks of culture on the conversion medium containing 5 mg litre-1 (23 µM) kinetin. Secondary somatic embryogenesis could also be obtained directly from the hypocotyls of mature primary somatic embryos cultured on the conversion medium. In our experimental system, only minor problems were noted with browning of cultures.