Diverse genetic bases of Indian polyembryonic and monoembryonic mango (Mangifera indica L) cultivars.
Ravishankar K. V., Chandrashekara P., Sreedhara S. A., Dinesh M. R., Lalitha Anand, Saiprasad G. V. S.
Author Affiliation: Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore 560 089, India.
87 : 870-871
Abstract : The genetic relatedness among 10 polyembryonic and monoembryonic cultivars each traditionally grown in the west coast of southern India were determined using random amplified polymorphic DNA (RAPD) markers. Eight mango cultivars from each of these groups were used for chloroplast DNA RFLP analysis. The primers ORF 106-rbcL and GIF-GI460 were used. All the products were restriction-digested with enzymes. Data from RAPD markers and chloroplast DNA RFLP markers were used for cluster analysis and principal component analysis (PCA), separately. Dendrogram analysis of RAPD and chloroplast DNA RFLP data clearly grouped the cultivars into 2 based on embryo types, i.e. monoembryonic and polyembryonic. In both genomic DNA and chloroplast DNA RFLP analyses, the grouping of cultivars based on their embryo types indicates that monoembryonic and polyembryonic types of Indian mango cultivars have a different genetic base. These results suggest that the polyembryonic types might have been introduced from other parts of southeast Asia and are unlikely to have originated from India.