Induction of somatic embryogenesis and plant regeneration of 'Ataulfo' mango (Mangifera indica).
Rivera-Domínguez M., Manzanilla-Ramírez M. A., Robles-González M., Gómez-Lim M. A.
Author Affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. Apdo. Postal 1735, Hermosillo 83000, Sonora, Mexico.
Plant Cell, Tissue and Organ Culture 79 : 101-104
Abstract : A study was conducted to establish a somatic embryogenesis system for mango cv. Ataulfo for a large-scale clonal propagation and eventually for genetic manipulation. Nucellar explants from immature fruits of Ataulfo, 3-4 cm long, were used. The process had a very low efficiency (10%). However, Ataulfo was more embryogenic than other cultivars such as Tommy Atkins or Haden. Overall, the process from nucellus to plantlet stage took 5 months. Twelve regenerated plants were obtained and are currently growing in the greenhouse. The plants appear to be normal and free of abnormalities.