Enzymatic characterization and crossed pathogenicity of Colletotrichum spp. associated with postharvest diseases.
Lima Filho R. M., Oliveira S. M. A., Menezes M.
Author Affiliation: Departamento de Agronomia, Área de Fitossanidade, Universidade Federal Rural de Pernambuco, CEP 52171-900, Recife, PE, Brazil.
Fitopatologia Brasileira 28 : 620-625
Abstract : Anthracnose caused by Colletotrichum spp. may result in great losses in field productions and postharvest conditions for several cultures and their products. The objective of this study was to test the pathogenicity of C. gloeosporioides isolates from cashew (Anacardium occidentale) (CAJ), mango (Mangifera indica) (MG), papaya (Carica papaya) (MM) and passion fruit (Passiflora edulis) (MR) and C. musae from banana (Musa spp.) (BAJ) to evaluate the production of extra cellular hydrolytic enzymes (amylolytic, cellulolytic, lypolytic and proteolytic) by the isolates on specific solid culture media; and to detect electrophoretic patterns of total proteins and isoenzymes (?-esterase, ?-esterase, acidic phosphatase and leucine aminopeptidase [cytosol aminopeptidase]). In crossed pathogenicity tests, all Colletotrichum spp. isolates produced necrotic and depressed lesions on fruits, except on passion fruit, which was susceptible only to the MR isolate. The isolates of C. gloeosporioides produced amylolytic, lypolytic, proteolytic, and cellulolytic enzymes, and C. musae did not produce any detectable cellulase. Electrophoretic analysis of total proteins and isoenzymes showed variations in the number and position of the bands among all isolates in the systems used, except for leucine aminopeptidase, which was monomorphic.