In vitro development and convertibility of nucellar embryos of Mangifera indica L. Bappakai, a dwarfing rootstock.
Chaturvedi H. C., Sharma M., Agnihotri S., Sharma A. K., Jain M., Chourasia A., Kidwai N. R., Gupta P.
Author Affiliation: Tissue Culture Laboratory, National Botanical Research Institute, Lucknow - 226 001, India.
Plant Cell Biotechnology and Molecular Biology 5 : 7-14
Abstract : Embryos differentiated from the nucellus of young ovular halves of Mangifera indica cv. Bappakai, an important dwarfing rootstock cultivar, on BM1 medium supplemented with 0.25 mg benzyladenine and 1 mg NAA/litre. Their proliferation was achieved in the medium containing 0.25 mg N6-(2-isopentenyl)adenine and 0.5 mg NAA/litre, with a decline in magnitude of embryo proliferation in long-term cultures. For near-synchronized development, maturation, germination (visible plumule with developed root) and convertibility (plantlet formation) of cotyledonary embryos, the liquid state of nutrient medium was required. The presence of 0.1 mg abscisic acid/litre together with 100 mg polyethylene glycol and 0.1 mg IAA/litre in the basal BM2 medium promoted development and maturation of embryos followed by their germination. The maximum percentage of germination obtained was 94% with embryos of 1.5 and 2 cm in length. However, only 80% of the germinated embryos converted into plantlets, with well-developed green leaves, on subculture in the same medium in the former case, while 76% in the latter. The in vitro-raised plantlets showed approximately 50% success in hardening.