Bio-assay technique to screen crude extract for controlling Colletotrichum gloeosporioides, post-harvest pathogen of mango anthracnose.
Jindawong P., Korpraditskul V., Ratanakreetakul C., Kittikup P., Kaewprasit J.
Author Affiliation: Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaengsean, Nakhon Pathom 73140, Thailand.
: 361-368
Abstract : Techniques for screening the efficacy of crude extract from Acorus calamus (at 0, 100, 500 and 1000 ppm) in inhibiting the growth of C. gloeosporioides [Glomerella cingulata] were evaluated. The highest rate of 1000 ppm showed 77.11% inhibition of vegetative growth in potato dextrose agar (PDA), while the inhibition zone under the paper disc diffusion technique could not be expressed. The extract at the highest concentration inhibited the growth by 94.5, 93.75, 79.75, 95.5, 62.37, 58.00, 67.81, 91.82 and 69.67% on water agar (WA), WA + rose bengal (RB), PDA, PDA + RB, microcentrifuge tube, multiwell plates, concave slide, slide culture and mango leaf, respectively. The concave slide technique was the best for screening the effect of the extract on the spore germination.